Investigating Size, Zeta Potential, and Molecular Weight and Evaluating Stability of BSA Solution

Three light scattering technologies, i.e., DLS, ELS, and SLS, are incorporated in the BeNano 90 Zeta to enable the measurements of size, zeta potential, and molecular weight, respectively. In this application note, the sizes of BSA in three dispersants are measured, showing the size trend when using different types of dispersants. Then, the molecular weight Mw of BSA is obtained by the BeNano 90 Zeta and shows excellent agreement with the Mw provided by the GPC system. Finally, by utilizing kD, zeta potential, and A2, the stabilities of BSA protein in different dispersants were successfully evaluated and sorted.

Product:

BeNano Series

Industry:

Biologics

Sample:

Proteins

Measurement Type:

Zeta Potential   

Measurement Technology:

Electrophoretic Light Scattering